Browsing by Author "Stashenko, Elena E."
Now showing 1 - 20 of 35
Results Per Page
Sort Options
Publication Acoplamiento molecular in sillico de los componentes de aceites esenciales con las proteínas del virus del dengue(2013) Pájaro Castro, Nerlis; Olivero Vebel, Jesús; Stashenko, Elena E.Dengue virus (DENV) is an arboviral infectious agent, this currently has no specific treatment. The main objective of this work was to evaluate the in silico molecular docking of the major components of essential oils with anti-dengue reported with DENV proteins. For this purpose, proteins were downloaded from Protein Data Bank (PDB): C, prM, E, NS3, NS5 polymerase and NS5 methyltransferase. Components of essential oils, previously optimized, were coupled using AutoDock Vina with proteins prepared and optimized. Theoretical affinity values for the components of the essential oils on protein varied between -4.0 and -7.3 kcal/mol. The molecules α-copaene, β-bourbonene, germacrene D, spathulenol, β-caryophyllene, caryophyllene oxide and (+)-epi-bicyclosesquiphellandrene, all of nature sesquiterpene, were those that showed the greatest interaction with the proteins studied. The majority of the interactions between these molecules and proteins were DENV hydrophobic type. This study computational has permitted infer that all dengue virus proteins are potential targets for the concomitant docking of various compounds of essential oils.Publication Análisis de fragancias florales por GC-MS(2013) Stashenko, Elena E.; Martínez Morales, Jairo RenéPublication Análisis de la composición química y actividad antioxidante en especies de Piper spp. (Fam. Piperaceae) recolectadas en zonas aledañas al departamento de Arauca - Colombia(2013) Valenzuela, Elisa E.; Tafurt García, Geovanna; Stashenko, Elena E.Publication Analysis of essential oils isolated by steam distillation from Swinglea glutinosa fruits and leaves(2015-04-21) Stashenko, Elena E.; Martínez Morales, Jairo René; Medina, José Domingo; Durán, Diego CamiloEssential oils obtained by steam distillation from Swinglea glutinosa (Blanco) Merr. fruit peel or leaves were analyzed by gas chromatography with flame ionization and mass spectrometry detection systems. A total of sixtyseven compounds were found, sixty-four of which were common to both oils, although present in different relative amounts. The main component of the essential oils obtained from S. glutinosa fruit peel or leaves was trans-nerolidol (19.1% and 28.4%, respectively). Sesquiterpene hydrocarbons (50%) and their oxygenated analogues (35%) were the most abundant compound types in the leaf oil. Monoterpene hydrocarbons (51%) were the principal compound family found in the oil obtained from fruit peels.Publication Cadena bioingredientes cosmética y aseo – Resumen ejecutivo(2015) Stashenko, Elena E.Publication Caracterización química y evaluación de la actividad antioxidante de extractos de flor de Jamaica (Hibiscus sabdariffa L.) obtenidos por diferentes técnicas de aislamiento(2014) Mejía Medina, Jesica Julieth; Stashenko, Elena E.Publication Chemical composition and bioactivity of Piper auritum and P. multiplinervium essential oils against the red flour beetle, Tribolium castaneum (Herbst)(2014) Caballero Gallardo, Karina; Olivero Vebel, Jesús; Pino Benítez, Nayive; Stashenko, Elena E.Stored grain insects have shown resistance to synthetic insecticides, fact that has promoted the use of vegetable species for integrated pest management. Piper auritum and P. multiplinervium are two plants from the Piperaceae family present in the department of Chocó, Colombia, one of the most important hot spots of biodiversity in the world. This study was conducted to determine the repellent activity and toxicity of essential oils (EOs) isolated from these plants against Tribolium castaneum, using the area preference and contact toxicity methods, respectively. P. auritum EO presented greater repellency than P. multiplinervium, the first showed 100% lethality at minimum tested exposure period (24 h) whereas the second reached 16% at 72 h. EOs were analyzed by gas chromatography-mass spectrometry. P. auritum major components were safrole (93.2%) and miristicine (4.3%), whereas for P. multiplinervium were β-elemene (9.0%), trans-β- caryophyllene (5.3%) and caryophyllene oxide (4.1%). It is speculated that the repellent effect of P. auritum may be related to its safrole content, a known repellent. These results evidenced Piper species could be used for development of repellents against T. castaneum.Publication Chemical Composition of Salvia aratocensis Extract obtained by Supercritical Fluid Extraction and its Antigenotoxicity against Ultraviolet Radiation-Induced DNA Damage(2015) Fuentes Lorenzo, Jorge Luis; Stashenko, Elena E.; Quintero Ruiz, NathaliaSalvia is a cosmopolitan and important genus, widely used in flavoring and traditional medicine for treatments of respiratory and gastrointestinal diseases. The present work evaluated the chemical composition of Salvia aratocensis extract obtained by supercritical CO2 extraction, as well as its DNA protective effect against ultraviolet radiation (UVR)-induced genotoxicity. S. aratocensis extract composition was determined by gas chromatography-mass spectrometry (GCMS). The antigenotoxic effect of S. aratocensis extract was assayed in co-incubation procedures using the SOS chromotest in Escherichia coli PQ37 cells. The major components of the plant extract were α-cadinol (31.8%), 1,10-epi-cubenol (12.9%), trans-β-caryophyllene (7.7%), -cadinene (6.6%), and squalene (6.5%). S. aratocensis extract protected bacterial cells against UVR-induced genotoxicity at doses between 0.063 and 1 mg/mL. The results were discussed in relation to the DNA protective potential of the S. aratocensis extract and its major constituents.Publication Chemical composition of the essential oil of Morina longifolia wall. Leaves(2013) Kumar, Arvind; Varsheney, Vinay K.; Rawat, Mohan Singh M.; Martínez Morales, Jairo René; Stashenko, Elena E.Essential oil from the leaves of Morina longifolia was isolated (yield 1.43%) using the hydro-distillation method and analysed for its chemical composition by capillary GC-FID and GC-MS. The oil showed the presence of 47 compounds representing 82.29 % area of total chromatogram. The oil was dominated by sesquiterpenes amounting to 53.02% with germacrene D (20.74%), α-cadinol (4.09%)l, and germacrene D-4-ol (3.73%) as major constituents. The composition and characteristics odor of the oil is indicative of its use in fragrance applications.Publication Chromatographic and mass spectrometric characterization of essential oils and extracts from Lippia (Verbenaceae) aromatic plants(2012-10-05) Stashenko, Elena E.; Martínez Morales, Jairo René; Cala, Mónica P.; Durán, Diego Camilo; Caballero, DeyaniraAnalytical methodologies based on GC and HPLC were developed for the separation and quantification of carnosic acid, ursolic acid, caffeic acid, p-coumaric acid, rosmarinic acid, apigenin, luteolin, quercetin, kaempferol, naringenin, and pinocembrin. These methods were used to characterize essential oils and extracts obtained by solvent (methanol) and by supercritical fluid (CO2) extraction from stems and leaves of Lippia (Verbenaceae family) aromatic plants (Lippia alba, Lippia origanoides, Lippia micromera, Lippia americana, Lippia graveolens, and Lippia citriodora). Supercritical CO2 extraction isolated solely pinocembrin and narigenin from three L. origanoides chemotypes. Solvent extracts possessed a more varied composition that additionally included apigenin, quercetin, and luteolin. Solvent extraction afforded higher overall flavonoid yields from all species in comparison with supercritical CO2 extraction. Pinocembrin was determined in L. origanoides extract at a concentration of 30 mg/g of plant material, which is more than ten times higher than the amount at which polyphenols are regularly found in aromatic plant extracts.Publication Contenido de compuestos fenólicos y capacidad antioxidante de extractos de mora (Rubus glaucus Benth) obtenidos bajo diferentes condiciones(2014-09-18) Rojas Llanes, Jennifer Pilar; Martínez Morales, Jairo René; Stashenko, Elena E.Publication Diseño experimental fraccionado para la extracción de antocianinas de flores de Hibiscus spp.(2014) Stashenko, Elena E.Publication Publication Essential oils from plants of the genus Cymbopogon as natural insecticides to control stored product pests(2015-04) Hernandez Lambraño, Ricardo; Pájaro Castro, Nerlis; Caballero Gallardo, Karina; Stashenko, Elena E.; Olivero Vebel, JesúsThe repellent and fumigant activities of essential oils (EOs) isolated from plants of the Cymbopogon genus against adults of Oryzaephilus surinamensis (L.) and Sitophilus zeamais Motsch were investigated. Cymbopogon martinii was the most toxic EO with LC50 values of 37.2 and 159 mL/L air for O. surinamensis and S. zeamais, respectively. Cymbopogon nardus EO showed greatest repellent activity with RC50 values of 0.03 and 0.04 mL/cm2, respectively, for examined pests. All oils were more effective as repellents than the commercial product IR3535, and O. surinamensis was more sensitive than S. zeamais to the EOs. These results suggest that EOs isolated from plants of the genus Cymbopogon can play an important role in stored grain protection and reduce the risks associated with the use of synthetic insecticides.Publication Estudio comparativo de la composición química y la actividad antioxidante de los aceites esenciales de algunas plantas del género Lippia (Verbenaceae) cultivadas en Colombia(2014) Stashenko, Elena E.; Martínez Morales, Jairo René; Durán, Diego Camilo; Córdoba, Yuri; Caballero, DeyaniraVarious results obtained from research on essential oils of vegetal aromatic species growing in Colombia are presented. In order to offer good scientific support to the essential oils agro-industry, the need for investigating a wide variety of topics is emphasized. Results of the chemical characterization and the antioxidant activity evaluation of 12 essential oils of 7 Lippia species are presented. The determination of the chemical composition of 12 essential oils of plants from genus Lippia, using gas chromatography coupled to mass spectrometry, showed that the most abundant and frequent constituents are oxygenated substances. The compositional diversity is manifested in optical isomers, in the existence of chemotypes with few substances in common, and in the diverse radical trapping modes, as evidenced by the ORAC and TEAC antioxidant capacity evaluations. While limonene and linalool were found in all the oils, 19 out of the 102 identified constituents of the set of oils studied were found in only one instance. The studied genus Lippia essential oils showed an antioxidant capacity higher than that exhibited by α-tocopherol or BHT, which are commonly used as antioxidants in commercial products for human consumption. Several species had a relatively high (1,5 – 4,4%) essential oil extraction yield, which together with their high antioxidant capacity convert these oils into good candidates to replace synthetic antioxidants.Publication Estudio de la capacidad de protecciòn de los aceites esenciales de Pogostemon cablin, Rosmarinus officinalis, Salvia officinalis, y Thymus vulgaris contra la oxidación lipídica en un jabón líquido(2013) García Domínguez, María Emilia; Stashenko, Elena E.; Martínez Morales, Jairo RenéPublication Estudio de la composición química de los aceites esenciales de seis especies vegetales recolectadas en Sucre y Bolívar, Departamento de Santander, Colombia(2013) Santos Quitián, Giohanna Katherine; González Mateus, Elizabeth; Stashenko, Elena E.; Martínez Morales, Jairo RenéPublication Estudio de la composición química de los metabolitos secundarios de Satureja viminea L., obtenidos por diferentes métodos de extracción y determinación de su capacidad antioxidante(2014-12-05) Gutiérrez Avella, Dora Marina; Stashenko, Elena E.Publication Evaluación de condiciones de operación de un destilador prototipo para la extracción de aceites esenciales que usa como combustible material vegetal residual(2014) Serrano Rivera, Rossy Jacqueline; Tavera Reyes, Camilo Andrés; Stashenko, Elena E.; Martínez Morales, Jairo René