Publication: Gluconacetobacter diazotrophicus in tropical tomato (Solanum lycopersicum L.) and sugarcane crops (Saccharum officinarum L.) from the West Central region of Colombia
No Thumbnail Available
Date
2015
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Background and aims Gluconacetobacter diazotrophicus is a plant growth-promoting bacterium (PGPB) that is endophytic and associated with sugarcane in Brazil. This study aims to determine the presence of G. diazotrophicus bacteria in sugarcane and tomato crops in Colombia. Methods Bacteria were isolated from roots, stems and leaf tissues and identified using phenotypic and biochemical evaluations and PCR assays. Isolates were characterized by their production of indole compounds, nitrogenase activity, and phosphorus solubilization. Results 100% of isolates obtained solubilize phosphorus, produce indole compounds in the presence of tryptophan, and exhibit nitrogenase activity. The isolates from sugarcane that were selected belong to the species G. diazotrophicus. Conclusions These results constitute the first report confirming the natural presence of G. diazotrophicus in Colombia. This finding has ecological significance and will advance research towards identifying the potential use of this bacterium in regional crops.