Publication:
Melanopsin-Mediated Transduction in Unconventional photoreceptors.

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dc.contributor.authorGomez, María del Pilar
dc.contributor.corporatenameUniversidad Nacional de Colombiaspa
dc.contributor.corporatenameCentro Internacional de Física (CIF) (Colombia)spa
dc.contributor.researchgroupCOL0022691 - Biofisica y Biologia de Membranas
dc.contributor.researchgroupCOL0063760 - Biofísica de la Señalización Celular
dc.contributor.researchgroupCOL0086143 - Neurofisiologia Celular
dc.coverage.projectdates2012-2014spa
dc.coverage.spatialColombia
dc.date.accessioned2019-10-30T15:36:18Z
dc.date.accessioned2020-12-18T00:36:15Z
dc.date.available2019-10-30T15:36:18Z
dc.date.available2020-12-18T00:36:15Z
dc.date.issued2014-04
dc.description.abstractEl proyecto permitió establecer por primera vez que en retina de pollo una población de células de la capa nuclear interna poseen fotosensibilidad intrínseca. Este organismo se plantea entonces como un modelo sumamente atractivo no solo para esclarecer los mecanismos de señalización de la melanopsina sino para aportar nuevos elementos a la historia evolutiva de la fototransducción. (Apartes del texto).spa
dc.format.extent23 páginas.spa
dc.identifier.instnameColcienciasspa
dc.identifier.reponameRepositorio Colcienciasspa
dc.identifier.repourlhttp://colciencias.metabiblioteca.com.cospa
dc.identifier.urihttps://colciencias.metadirectorio.org/handle/11146/39574
dc.language.isospaspa
dc.relation.ispartofseriesInforme;
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.accessrightshttp://purl.org/coar/access_right/c_abf2spa
dc.rights.creativecommonshttps://creativecommons.org/licenses/by/4.0/spa
dc.subject.armarcPercepción visual
dc.subject.proposalMelanopsinaspa
dc.subject.proposalPigmento visualspa
dc.subject.proposalCélulas ganglionares de la retinaspa
dc.subject.proposalFototransducciónspa
dc.subject.proposalPatch Clampen
dc.subject.proposalSeñalizaciónspa
dc.subject.proposalVisiónspa
dc.titleMelanopsin-Mediated Transduction in Unconventional photoreceptors.spa
dc.typeInforme de investigaciónspa
dc.type.coarhttp://purl.org/coar/resource_type/c_18wsspa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/reportspa
dc.type.redcolhttps://purl.org/redcol/resource_type/PIDspa
dc.type.versioninfo:eu-repo/semantics/submittedVersionspa
dc.type.versionhttp://purl.org/coar/version/c_71e4c1898caa6e32spa
dc.type.versioninfo:eu-repo/semantics/submittedVersionspa
dcterms.audienceEstudiantes, Profesores, Comunidad científica colombiana, etc.spa
dspace.entity.typePublication
oaire.awardnumber222852128276spa
oaire.funderidentifier.colciencias443-2011
oaire.fundernameDepartamento Administrativo de Ciencia, Tecnología e Innovación [CO] Colcienciasspa
oaire.objetivesGeneral Objectives : The long-term goals of this project are to elucidate the cellular and molecular underpinnings of light-sensing in native cells that utilize the photopigment melanopsin. The chicken retina presents itself as an interesting and valuable system to study melanopsin transduction due to the abundant expression of this protein in the inner nuclear layer possibly in bipolar and horizontal cells. The functional role of melanopsin in these retinal layer is completely unknown which opens the possibility of uncovering a completely novel light sensor. More important this organism may provide clues on the evolution of different lineages of photoreceptors and their respective signaling cascades.en
oaire.objetivesSpecific Objectives : 1. Develop a preparation of functionally viable cells from the retina of chicken embryos, isolated by means of enzymatic treatment and mechanical dispersion.en
oaire.objetivesSpecific Objectives : 2. Determine which of the defined cell types present in the inner nuclear layer of chicken embryo retinas express melanopsin.en
oaire.objetivesSpecific Objectives : 3. Use a fluorescence assay to establish the presence of retinal in melanosin-expressing cells, which would suggest that melanosin forms a functional photopigment.en
oaire.objetivesSpecific Objectives : 4. Utilize patch-clamp recordings to examine whether melanopsin-expressing cells are intrinsically photosensitive.en
oaire.objetivesSpecific Objectives : 5. Detect and localize key signaling molecules of the phosphoinositide pathway in the chicken retina, using Western blot analysis, and isolated-cell immunocytochemistry.en
oaire.objetivesSpecific Objectives : 6. Ascertain the functional role of key downstream elements of the cascade, by means of single-cell electrophysiology in conjunction with Ca fluorescence measurements and pharmacology.en

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