Publication:
Pangenome-wide and molecular evolution analyses of the Pseudonomas aeruginosa species

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col.comunidadvinculadaComunidad científica colombianaes_CO
col.contrato0020-2013es_CO
col.date.proyecto2015-08-10
col.programa.colcienciasPrograma Nacional de Biotecnologíaes_CO
col.tipo.espGeneración de contenidos impresos, radiales, audiovisuales, multimedia, virtuales y Creative Commonses_CO
dc.audienceEntidades gubernamentaleses_CO
dc.audienceInvestigadoreses_CO
dc.coverage.spatialColombiaes_CO
dc.creatorBenítez Páez, Alfonso
dc.creatorMosquera Rendón, Jeanneth
dc.creatorCárdenas Brito, Sonia
dc.creatorRestrepo Pineda, Eliana
dc.creatorCorredor Rodríguez, Mauricio
dc.creatorRada Bravo, Ana M.
dc.creator.corporativoCentro de Investigación y Desarrollo en Biotecnologíaes_CO
dc.creator.corporativoUniversidad de Antioquia, UdeAes_CO
dc.creator.corporativoUniversidad Cooperativa de Colombia, UCCes_CO
dc.creator.mailabenitez@cidbio.orges_CO
dc.date.accessioned2019-03-25T18:18:09Z
dc.date.available2019-03-25T18:18:09Z
dc.date.embargoEndinfo:eu-repo/date/embargoEnd/2024-01-31es_CO
dc.date.issued2015
dc.description.abstractBackground. Duck treatments and vaccine designs against the opportunistic human pathogen Pseudomonas aeruginosa have multiple issues, all associated with the diverse genetic traits present in this pathogen, ranging from multi-drug resistant genes to the molecular machinery for the biosynthesis of biofilms. Several candidate vaccines against P. aeruginosa have been developed, which target the outer membrane proteins; However, major issues arise when attempting to establish complete protection against this pathogen due to its presumably genotypic variation at the strain level. To shed light on this concern, we propose this study to assess the P. aeruginosa pangenome and its molecular evolution across multiple strains. Results the P. aeruginosa pangenome was estimated to contain almost 17,000 non-redundant genes, and approximately 15% of these constituted the core genome. Functional analyzes of the accessory genome indicated a wide presence of genetic elements directly associated with pathogenicity. An in-depth molecular evolution analysis revealed the full landscape of selection forces acting on the P aeruginosa pangenome, in which purifying selection drives evolution in the genome of this human pathogen. We also detected distinctive selection wide variety of outer membrane proteins, with the data supporting the concept of substantial genetic variation in proteins probably recognized antigens. Approaching the evolutionary information of genes under extremely positive selection, we designed a new multi-locus sequencing typing assay for and informative, rapid and cost-effective genotyping of P. Aeruginosa on a large scale.es_CO
dc.description.isprojectnoes_CO
dc.description.projectid5817-569-34856es_CO
dc.description.projectnameEstablecimiento de módulo funcionales de secuencia en familias de metiltransferasas de RNA y su uso en la búsqueda de genes de resistencia a antibióticos en patógenos humanoses_CO
dc.description.sponsorshipDepartamento Administrativo de Ciencia, Tecnología e Innovación [CO] Colcienciases_CO
dc.formatpdfes_CO
dc.format.extent37 páginases_CO
dc.identifier.bibliographicCitationContiene 45 referencias bibliográficas. Véase el documento adjuntoes_CO
dc.identifier.urihttp://repositorio.colciencias.gov.co/handle/11146/34126
dc.language.isoenges_CO
dc.relation.ispartofEstablecimiento de módulos funcionales de secuencia en familias de metiltransferasas de RNA y su uso en la búsqueda de genes de resistencia a antibióticos en patógenos humanos. La publicación completa está disponible en : <a href="http://repositorio.colciencias.gov.co/handle/11146/34125" target="blank">http://repositorio.colciencias.gov.co/handle/11146/34125</a>es_CO
dc.rightsinfo:eu-repo/semantics/embargoedAccesses_CO
dc.subject.spinesÁcido ribonucleicoes_CO
dc.subject.spinesSecuencia de aminoácidoses_CO
dc.subject.spinesBacteriases_CO
dc.subject.spinesBioquímicaes_CO
dc.titlePangenome-wide and molecular evolution analyses of the Pseudonomas aeruginosa specieses_CO
dc.title.alternativeBMC Gebomicses_CO
dc.typeOtroses_CO
dc.type.driverinfo:eu-repo/semantics/other.generacióndecontenidoses_CO
dc.type.hasversioninfo:eu-repo/semantics/allowedVersiones_CO
dspace.entity.typePublication

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