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Molecular Characterization of Resistance in Cassava (Manihot esculenta Crantz) to attack the green mite (Mononychellus tanojoa): a proteomics approach.

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dc.contributor.authorMontoya Lerma, James
dc.contributor.corporatenameUniversidad del Valle (Cali, Colombia)spa
dc.contributor.researchgroupCOL000748 - Investigaciones Entomológicas
dc.contributor.researchgroupCOL0072881 - Interacciones Tritroficas
dc.contributor.researchgroup000000 - Cassava Research Group - Plant Biotechnology
dc.coverage.projectdates2011-2015spa
dc.date.accessioned2020-10-26T20:39:47Z
dc.date.accessioned2020-12-17T23:56:09Z
dc.date.available2020-10-26T20:39:47Z
dc.date.available2020-12-17T23:56:09Z
dc.date.issued2015-01
dc.description.abstractScientists at CIAT and Universidad del Valle have identified wild cassava accessions resistant to green mite. In order to foster the introgression of the resistance trait into farmer-preferred cultivars, the research project proposes to undertake a molecular characterization of the mechanisms underlying the green mite resistance in the wild cassava accessions. The recent release of the cassava genome allows the implementation of advanced label-free protein quantification to characterize the proteome and its modulation upon arthropod attacks. The system, green mite - cassava, will be investigated with the resistant and susceptible accessions for the system. In addition, it is intended to evaluate whether the resistance of the selected cassava accessions is induced or constitutive. To do so, a comparison of pest performance on (1) plants previously unexposed to arthropod attack with (2) plants previously challenged by arthropods, will be performed. For the complete experimental set, sampling at several key time points during arthropod establishment and development will allow deciphering the constitutive and inducible proteome differences. The label-free protein quantification will generate a list of gene candidates whose expressions relate to arthropod resistance for the system under investigation. Specific qPCR primers will be developed and transcript quantification of the selected gene candidates will be performed on the leaf material sampled during the aforementioned entomological experiments. For each candidate gene, the following transcript measurement will be generated: relative expression between susceptible and resistant accessions, time course expression analysis, and expression level.spa
dc.format.extent22 páginas.spa
dc.identifier.instnameColcienciasspa
dc.identifier.reponameRepositorio Colcienciasspa
dc.identifier.repourlhttp://colciencias.metabiblioteca.com.cospa
dc.identifier.urihttps://colciencias.metadirectorio.org/handle/11146/39165
dc.language.isospaspa
dc.relation.ispartofseriesInforme;
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.accessrightshttp://purl.org/coar/access_right/c_abf2spa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.accessrightshttp://purl.org/coar/access_right/c_abf2spa
dc.rights.creativecommonshttps://creativecommons.org/licenses/by/4.0/spa
dc.subject.proposalCassavaspa
dc.subject.proposalGreen mitespa
dc.subject.proposalModulationspa
dc.subject.proposalProteomicsspa
dc.subject.proposalResistance/Tolerancespa
dc.titleMolecular Characterization of Resistance in Cassava (Manihot esculenta Crantz) to attack the green mite (Mononychellus tanojoa): a proteomics approach.spa
dc.typeInforme de gestiónspa
dc.type.coarhttp://purl.org/coar/resource_type/c_93fcspa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/reportspa
dc.type.redcolhttps://purl.org/redcol/resource_type/PIDspa
dc.type.versioninfo:eu-repo/semantics/submittedVersionspa
dc.type.versionhttp://purl.org/coar/version/c_71e4c1898caa6e32spa
dc.type.versioninfo:eu-repo/semantics/submittedVersionspa
dc.type.versionhttp://purl.org/coar/version/c_71e4c1898caa6e32spa
dcterms.audienceEstudiantes, Profesores, Comunidad científica colombiana, etc.spa
dspace.entity.typePublication
oaire.awardnumber110652128281spa
oaire.funderidentifier.colciencias2010-521
oaire.fundernameDepartamento Administrativo de Ciencia, Tecnología e Innovación [CO] Colcienciasspa
oaire.objetivesMolecular characterization of resistance/tolerance to green mite, through the identification of proteins in cassava that exhibit an expression patterns correlated with resistance/tolerance to the pest.spa

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