Publication:
Lippia origanoides supercritical fluid extract and its major constituent pinocembrin diminish ultraviolet radiation-induced DNA damage and modulate cell division in Escherichia coli

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col.comunidadvinculadaComunidad científica colombianaes_CO
col.contrato0572-2012es_CO
col.date.proyecto2018-01
col.programa.colcienciasProgramas de CT+I Ejecutados por Redes de Conocimientoes_CO
col.tipo.espGeneración de contenidos impresos, radiales, audiovisuales, multimedia, virtuales y Creative Commonses_CO
dc.audienceAdministradores de ciencia y tecnologíaes_CO
dc.audienceInvestigadoreses_CO
dc.coverage.spatialColombiaes_CO
dc.creatorRey Castellanos, Natalia
dc.creatorStashenko, Elena E.
dc.creatorEstévez Castro, Carlos F.
dc.creator.corporativoBio-Red-CO-CENIVAMes_CO
dc.creator.corporativoUniversidad Industrial de Santander, UISes_CO
dc.creator.mailjfuentes@uis.edu.coes_CO
dc.date.accessioned2019-04-01T03:51:45Z
dc.date.available2019-04-01T03:51:45Z
dc.date.embargoEndinfo:eu-repo/date/embargoEnd/2024-01-31es_CO
dc.date.issued2015-09
dc.description.abstractThe objective of this work was to evaluate DNA protective properties against UVR-induced genotoxicity of an L. origanoides extract obtained by CO2-supercritical fluid extraction and its major constituent, pinocembrin. L. origanoides extract composition was determined by gas chromatography-mass spectrometry (GC-MS) and its antigenotoxic effect using the SOS chromotest. Fluorescence microscopy analysis was also used in cell treatments in order to determine interferences with cell division and different nucleoid shapes categories were measured. The principal constituents of the plant extract were pinocembrin (54.9%), trans-β- caryophyllene (10.9%), carvacrol (6.3%), p-cimene (6.2%), thymol (4.6%), and 1,8-cineole (3.5%). L. origanoides extract protected bacterial cells against UVR-induced genotoxicity at doses between 0.016 and 1 mg/mL; this effect was related to its major constituent pinocembrin. Fluorescence microscopy analysis showed that pinocembrin modulated the cell division and nucleoid shape in E. coli cells. The results were discussed in relation to the DNA protective potential of the L. origanoides species.es_CO
dc.description.isprojectnoes_CO
dc.description.projectid5507-543-31904es_CO
dc.description.projectnamePrograma: Bioprospección y desarrollo de ingredientes naturales para las industrias cosmética, farmacéutica y de productos de aseo con base en la biodiversidad colombianaes_CO
dc.description.sponsorshipDepartamento Administrativo de Ciencia, Tecnología e Innovación [CO] Colcienciases_CO
dc.formatpdfes_CO
dc.format.extent19 páginases_CO
dc.identifier.bibliographicCitationContiene 8 referencias bibliográficas. Véase documento adjuntoes_CO
dc.identifier.urihttp://repositorio.colciencias.gov.co/handle/11146/34183
dc.language.isoenges_CO
dc.relation.ispartofPrograma: Bioprospección y desarrollo de ingredientes naturales para las industrias cosmética, farmacéutica y de productos de aseo con base en la biodiversidad colombiana. La publicación completa está disponible en : <a href="http://repositorio.colciencias.gov.co/handle/11146/34163" target="blank">http://repositorio.colciencias.gov.co/handle/11146/34163</a>es_CO
dc.rightsinfo:eu-repo/semantics/embargoedAccesses_CO
dc.sourceJournal: Natural Product Researches_CO
dc.subject.keywordAntigenotoxicity
dc.subject.keywordSOS Chromotest
dc.subject.keywordUltraviolet radiation
dc.subject.keywordLippia origanoides
dc.subject.keywordSupercritical fluid extraction
dc.subject.keywordPinocembrin
dc.subject.lembEscherichia colies_CO
dc.subject.lembBiología vegetales_CO
dc.subject.lembCromatografía de gaseses_CO
dc.subject.spinesBiología moleculares_CO
dc.subject.spinesAceites esencialeses_CO
dc.subject.spinesBioquímica vegetales_CO
dc.subject.spinesTecnología químicaes_CO
dc.subject.spinesCatalizadoreses_CO
dc.titleLippia origanoides supercritical fluid extract and its major constituent pinocembrin diminish ultraviolet radiation-induced DNA damage and modulate cell division in Escherichia colies_CO
dc.typeOtroses_CO
dc.type.driverinfo:eu-repo/semantics/other.generacióndecontenidoses_CO
dc.type.hasversioninfo:eu-repo/semantics/draftes_CO
dspace.entity.typePublication

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