Publication: Anaerobic solvent - producing bacteria from colombian sources, fermetation of biomass and isolation of cellulolytic strains
Loading...
Date
2004-08-31
Authors
Montoya CastaƱo, Dolly
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
A new type of solvent producing bacteria was isolated from Colombian soil. They are related to Clos-tridium butyricum, hitherto unknown to produce solvents. This opens new perspectives for solvent production. In the light of the new taxonomic position, the strains will be analysed for i-propanol, propane-diol and other higher value solvents production potential. Selected strains will be used for biomass conversion to solvents and the fermentation process will be optimised.
The recently published genomic DNA sequence of C. acetobutylicum has disclosed the presence of a complete, but inactive cellulose operon. This group of bacteria has there for the potential for crystalline cellulose hydrolysis, opening a new perspective to investigate group I clostridia (like all solventogenic clostridia including the new strains) for their ability to use of a broader range of abundant biomass for solvent production. In addition to the existing solvent pro-ducers, new strains will be screened by selecting anaerobic soil bacteria producing solvents from na-tive cellulose. Molecular ge-netics' methods will be applied to the strains to identify plasmids and genes for solvent production and cellulolysis as a prerequisite for future genetical manipulation. Taxo-nomic and phylogenetic studies will be performed to investigate cellulolytic soil bacteria biodiversity. By investigating the cellulolytic genes, cellulo-some gene evolution will be addressed.