Publication:
Rosuvastatin inhibits IL-8 and IL-6 production in human coronary artery endothelial cells stimulated with Aggregatibacter actinomycetemcomitans

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col.comunidadvinculadaComunidad de odontólogos colombianoses_CO
col.contrato0261-2010es_CO
col.date.proyecto2015-04
col.programa.colcienciasPrograma nacional de ciencias y tecnologías de la saludes_CO
col.tipo.espGeneración de contenidos impresos, radiales, audiovisuales, multimedia, virtuales y Creative Commonses_CO
dc.audienceProfesoreses_CO
dc.audienceInvestigadoreses_CO
dc.coverage.spatialBogotáes_CO
dc.creatorGualtero Escobar, Diego Fernando
dc.creatorViafara, Sergio
dc.creatorMorantesa, Sandra J.
dc.creatorGonzález Duque, Octavio Alberto
dc.creatorLafaurie Villamil, Gloria Inés
dc.creator.corporativoUniversidad de Kentucky, UKes_CO
dc.creator.corporativoUniversidad El Bosquees_CO
dc.creator.corporativoUniversidad Nacional de Colombia, UNALes_CO
dc.creator.mailinstitutouibo@gmail.comes_CO
dc.date.accessioned2018-10-04T17:56:26Z
dc.date.available2018-10-04T17:56:26Z
dc.date.embargoEndinfo:eu-repo/date/embargoEnd/2024-01-31es_CO
dc.date.issued2015
dc.description.abstractAggregatibacter actinomycetemcomitans (A.a) is a Gram negative periodontopathogen that has been highly associated with endocarditis and atherosclerosis. However, the potential mechanisms by which A.a could be contributing to atherosclerosis remain unclear. The purpose of this study was to determine the effect of purified LPS from A.a (Aa-LPS) on the expression of pro-inflammatory molecules (i.e., adhesion molecules, Toll-like receptors and cytokines/chemokines) associated with the pathogenesis of atherosclerosis in human coronary artery endothelial cells (HCAECs), as well as evaluating the potential of Rosuvastatin (RSV) for inhibiting the A.a-induced endothelial responses. HCAECs were stimulated with purified A. a-LPS and cytokine expression levels determined by qPCR and flow cytometry, and TLR2 and TLR4 expression evaluated by ELISA fluorometric assay. The effect of RSV in Aa-LPS-induced pro-inflammatory responses was also studied using similar experimental approaches. A. a-LPS increased the expression of IL-6, IL-8, and TLR2 in HCAECs. No effects in the expression of adhesion molecules were observed. Aa-induced IL-6 and IL-8 production was inhibited by RSV particularly at higher doses. These results suggest that Aa-LPS plays a role in pro-inflammatory endothelial responses that could be contributing to the atherosclerotic process, and the use of statins (i.e., RSV) could be reducing the likelihood for Aa-induced pro-atherosclerotic endothelial responses.es_CO
dc.description.isprojectnoes_CO
dc.description.projectid1308-519-28960es_CO
dc.description.projectnameInducción de disfunción endotelial in vitro por lipopolisacarido de bacterias periodontopaticas e inhibición de la inflamación por resolvina (rvd1) y estatina (rosuvastatina)es_CO
dc.description.sponsorshipDepartamento Administrativo de Ciencia, Tecnología e Innovación [CO] Colcienciases_CO
dc.formatpdfes_CO
dc.format.extent36 páginases_CO
dc.identifier.urihttps://repositorio.minciencias.gov.co/handle/20.500.14143/22042
dc.language.isoenges_CO
dc.relation.ispartofInducción de disfunción endotelial in vitro por lipopolisacarido de bacterias periodontopaticas e inhibición de la inflamación por resolvina (rvd1) y estatina (rosuvastatina). La publicación completa está disponible en : <a href="http://repositorio.colciencias.gov.co:80/handle/11146/22037" target="blank">http://repositorio.colciencias.gov.co:80/handle/11146/22037</a>
dc.rightsinfo:eu-repo/semantics/embargoedAccesses_CO
dc.source.bibliographicCitationContiene 50 referencias bibliográficas. Véase el documento adjuntoes_CO
dc.subject.keywordStatins
dc.subject.keywordAtherosclerosis
dc.subject.keywordInnate immune response
dc.subject.keywordActinomycetemcomitans
dc.subject.lembCavidades periodontaleses_CO
dc.subject.lembEncías -- Enfermedadeses_CO
dc.subject.spinesEnfermedades de la bocaes_CO
dc.subject.spinesBacteriologíaes_CO
dc.titleRosuvastatin inhibits IL-8 and IL-6 production in human coronary artery endothelial cells stimulated with Aggregatibacter actinomycetemcomitanses_CO
dc.typeOtroses_CO
dc.type.driverinfo:eu-repo/semantics/other.generacióndecontenidoses_CO
dc.type.hasversioninfo:eu-repo/semantics/acceptedVersiones_CO
dspace.entity.typePublication

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