Browsing by Author "Martignon Biermann, Stefania"

Now showing 1 - 14 of 14
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    Cartilla para odontólogos Diagnóstico diferencial entre fluorosis dental y caries
    (2015-11) Martignon Biermann, Stefania; Avila, Viviana; Castiblanco Rubio, Gina Alejandra; González Carrera, María Clara; Opazo Gutiérrez, Mario Omar; Martínez Mier, Esperanza Ángeles; Cury, Jaime Aparecido; Ruiz Carrizosa, Jaime Alberto; Silva Hermida, Blanca Cecilia
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    Diagnóstico diferencial entre caries y fluorosis: reproducibilidad entre examinadores en diagnostico visual y confirmacion histologica. (od124)
    (2014-10) Castiblanco Rubio, Gina Alejandra; Martignon Biermann, Stefania; Marín Gallón, Lina María; Martínez Mier, Esperanza Ángeles; Úsuga Vacca, Margarita V.
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    Diferencias en características demográficas, biológicas y sociales entre estadios de severidad de fluorosis dental en población preescolar y escolar de cuatro municipios del Huila
    (2015) Martignon Biermann, Stefania; Castiblanco Rubio, Gina Alejandra; Silva Hermida, Blanca Cecilia; Opazo Gutiérrez, Mario Omar; González Carrera, María Clara; Ávila, Viviana; Gamboa, Luis Fernando; Marín Gallón, Lina María; Cortés, Fabián; Ruiz Carrizosa, Jaime Alberto
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    Diseño de una cartilla informativa sobre fluorosis dental y uso del fluoruro dirigida a la población general
    (2014) Gómez Barajas, Carlos Eduardo; Lugo Angulo, Wendy Yolanny; Piñeros Bermúdez, Stephanie; Martignon Biermann, Stefania
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    Estandarización de la técnica de biopsia para determinación de fluoruro en esmalte dental
    (2015) Marín Gallón, Lina María; Castellanos, Jaime Eduardo; Andaló Tenuta, Livia Maria; Martignon Biermann, Stefania; Cury, Jaime Aparecido
    Introduction. Pre-eruptive incorporation of fluoride into dental enamel reflects its systemic exposition during amelogenesis, leading to the use of dental enamel as a biomarker of fluoride exposure. There are not reported studies in Colombia using the enamel biopsy technique, which allows the determination of the fluoride distribution pattern throughout dental enamel. Aim. To standardize the enamel biopsy technique and determine the fluoride content in the enamel of sound unerupted teeth. Methods. With ethical approval and informed consent forms, there were collected 5 third unerupted molar-teeth and of each tooth it were obtained two enamel blocks from the middle third of the crown, in a cross-sectional way. All surfaces were covered with acid-resistant wax leaving the external enamel surface exposed, which were treated with 0.5M HCl for 15, 30, 60, and 120 seconds under constant agitation. Fluoride concentration was determined in each acid extract by means of a fluoride ion-specific electrode and the mass of removed enamel was established (grams) after Pi analyses. The fluoride content in each layer of removed enamel was determined (μg F/g enamel) and in the total of removed enamel (μg F/cm3). Results: There was a fluoride distribution pattern from the surface to the inner enamel, being higher the fluoride concentration in the outer enamel surface (1663.49 ± 266.61 μg F/g enamel). Total fluoride concentration in the total of removed enamel was 1.02 ± 0.25 μg F/cm3. Conclusions: Enamel biopsy technique was standardized. There was found a fluoride distribution pattern from the surface to the inner enamel, similar to the data reported in the literature.
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    Geochemical characterization of fluoride and its relationship with the prevalence of enamel fluorosis in children in four municipalities of the department of Huila (Colombia)
    (2015) Martignon Biermann, Stefania; Opazo Gutiérrez, Mario Omar; Velásquez Riaño, Möritz; Orjuela Osorio, Iván Rodrigo; Ávila, Viviana; Martínez Mier, Esperanza Ángeles; González Carrera, María Clara; Ruiz Carrizosa, Jaime Alberto; Silva Hermida, Blanca Cecilia
    Fluoride is an element that affects teeth and bone formation in animals and humans. Though the use of fluoride is an evidence-based caries preventive measure, excessive ingestion can impair tooth development, mainly the mineralization of tooth enamel, leading to a condition known as enamel fluorosis. In this study, we investigated the relationship between the prevalence of enamel 2 fluorosis in children and the geochemical characterization of fluoride in four sentinel municipalities of the department of Huila, Colombia (Pitalito, Altamira, El Agrado and Rivera). The concentration of fluoride in drinking water, table salt, rocks and active sediment was evaluated by means of an ion selective electrode. Geochemical analyses were performed using X-Ray fluorescence. These revealed fluoride concentrations under 15 mg/kg in soil, rocks, and active sediment samples, not indicative of a significant delivery to the watersheds studied. The concentration of fluoride in table salt was found to be under the inferior limit (180-220 μg/g) established by the Colombian regulations. Likewise, exposure doses for fluoride water intake did not exceed the recommended total dose for all ages from 6 months. Although the evidence does not point out at rocks, soils, fluoride-bearing minerals, fluoridated salt and water, the hypothesis of these elements as responsible of the prevalence of enamel fluorosis cannot be discarded since, aqueducts might have been undergone significant changes overtime.
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    Higher Fluorosis Severity Makes Enamel Less Resistant to Demineralization
    (2016) Marín Gallón, Lina María; Cury, Jaime Aparecido; Andaló Tenuta, Livia Maria; Castellanos, Jaime Eduardo; Martignon Biermann, Stefania
    Fluorotic teeth could either be more resistant or more susceptible to the caries process than sound ones due to their higher enamel fluoride concentration and higher porosity (subsurface hypomineralization), respectively; however, there is no consensus on this subject. In this study, a total of 49 human unerupted third molars presenting Thylstrup and Fejerskov (TF) fluorosis scores 0–4 were used. Two enamel slabs were obtained from each tooth. The rest of the tooth crown was powdered, and the enamel was separated from the dentine. In purified powdered enamel, the calcium (Ca), inorganic phosphate (P i ), and fluoride (F) concentrations were determined. The F concentration gradient throughout the enamel and in the enamel, volume was determined in one slab. The other enamel slab was isolated with acid-resistant varnish, subjecting the exposed enamel surface half to a pH-cycling model to evaluate its demineralization resistance and to calculate the demineralization area. The nonexposed surface was used to determine the natural hypomin eralization area found in fluorotic enamel and normalize the demineralization data. The hypomineralization and demineralization areas were assessed by cross-sectional microhardness. For statistical analyses, the data for TF1 and 2, and for TF3 and 4 were pooled. Concentrations of powered enamel Ca and Pi were not significantly different (p > 0.05) among groups TF0, TF1–2 and TF3–4, but a higher F concentration was found in fluorotic enamel (p < 0.05). Highly fluorotic teeth (TF3–4) presented a greater hypomineralization subsurface area and demonstrated lower demineralization resistance than sound enamel (p < 0.05). The findings suggest that a higher severity of fluorosis makes enamel less resistant to the caries process due to its greater subsurface mineral area exposed to demineralization and deeper acid diffusion through the enamel.
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    Identification of proteins from human permanent erupted enamel
    (2015-09) Castiblanco Rubio, Gina Alejandra; Rutishauser, Dorothea; Ilag, Leopold L.; Martignon Biermann, Stefania; Castellanos, Jaime Eduardo; Mejía, Wilson
    Proteins from the extracellular matrix of enamel are highly specific and necessary for proper enamel formation. Most proteins are removed from the matrix by enamel proteases before complete mineralization is achieved; however, some residual protein fragments persist in the mineralized matrix of erupted enamel. So far, only amelogenin peptides obtained by traditional bottom-up proteomics have been recovered and identified in human permanent erupted enamel. In this study, we hypothesize that other enamel-specific proteins are also found in human permanent enamel, by analysing human erupted third molars. Pulverized enamel was used to extract proteins, and the protein extract was subjected directly to liquid-chromatography coupled to tandem mass spectrometry (LC-MS/MS) without a previous trypsindigestion step. Amelogenin and non-amelogenin proteins (ameloblastin and enamelin) were succesfully identified. The sequences of the naturally occurring peptides of these proteins are reported, finding in particular that most of the peptides from the amelogenin X-isoform come from the tyrosine-rich amelogenin peptide (TRAP) and that some were identified in all specimens. In conclusion, our LC-MS/MS method without trypsin digestion increased the coverage of identification of the enamel proteome from a few amelogenin peptides to a higher number of peptides from three enamel-specific proteins.
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    In vitro validation of Quantitative Light-induced Fluorescence (QLF) for the diagnosis of enamel fluorosis in permanent teeth
    (2015) Cuevas Espinosa, Diana Milena; Martínez Mier, Esperanza Ángeles; Masatoshi, Ando; Castiblanco Rubio, Gina Alejandra; Cortés, Fabián; Rincón Bermúdez, Claudia Milena; Martignon Biermann, Stefania
    The use of Quantitative Light-induced Fluorescence (QLF) for the diagnosis of enamel fluorosis has not been validated. This study aimed to validate QLF as a diagnostic tool for mild and moderate fluorosis in permanent teeth, comparing it to visual diagnosis and histological assessment completed using polarized light microscopy (PLM). The buccal surfaces of 139 teeth were visually classified using the Thylstrup and Fejerskov Index (TFI) into sound (TFI 0; n=17), mild (TFI 1-2; n=69) and moderate (TFI 3-4; n=43) fluorosis. Fluorosis was then assessed with QLF using the entire surface and a region of interest (ROI), identified as the most representative region of a fluorosis lesion. PLM images of longitudinal thin sections including the ROI were assessed for histological changes. Correlations among TFI, PLM, and QLF were determined. A ROC curve was conducted to determine QLF’s diagnostic accuracy when compared to the TFI and PLM assessments. This was used to assess the probability that the images were correctly ranked according to severity as determined by PLM and TFI. A positive correlation was found between QLF and PLM, and between QLF and TFI. QLF showed highest sensitivity and specificity for the diagnosis of mild fluorosis. There was also a strong agreement between TFI and PLM. The selection of a ROI resulted in a stronger correlation with TFI and PLM than when the entire surface was used. The study results indicate that defining a ROI for QLF assessments is a valid method for the diagnosis of mild and moderate enamel fluorosis.
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    Quantitative light fluorescence (QLF) assessment of mild and moderate enamel fluorosis in primary teeth
    (2015) Rincón Bermúdez, Claudia Milena; Martínez Mier, Esperanza Ángeles; Castiblanco Rubio, Gina Alejandra; Masatoshi, Ando; Cortés, Fabián; Cuevas Espinosa, Diana Milena; Martignon Biermann, Stefania
    Enamel fluorosis affects both permanent and primary teeth; however, there are few studies on primary teeth. Fluorosis in primary teeth is difficult to diagnose for mild and moderate cases. Quantitative light-induced fluorescence (QLF) has been used for enamel fluorosis on the permanent dentition, but there are no current studies on primary fluorotic teeth. The purpose of this study was to assess mild to moderate enamel fluorosis in primary teeth using QLF. The buccal surfaces of exfolicated and extracted primary teeth (n=113) were visually examined and classified using the Thylstrup & Fejerskov Index (TFI) for fluorosis into categories TFI-0 to TFI-4. Fluorescence images were acquired. Image analysis was performed using the complete tooth surface (S) and through the selection of a Region of Interest (ROI), which was determined as the region where the fluorosis lesions were more representative and with at least a minimum of adjacent sound area. Area (mm2), fluorescence loss (%) and ΔQ (mm2×%) values were obtained. The correlation between QLF variables and visual diagnosis was determined using the Kendall-tau coefficient. Sencitivity, specificity, and Receiver Operating Characteristic analysis was performed for QLF versus visual diagnosis. Moderate to strong positive correlation was found between QLF and visual diagnosis using the ROI. QLF showed greater sensitivity and specificity for the diagnosis of mild fluorosis than moderate fluorosis and with the ROI analysis than with S. QLF was a useful tool for the assessment of mild and moderate fluorosis in primary teeth using a ROI analysis technique.
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