Browsing by Author "Quintero Ruiz, Nathalia"

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    Chemical Composition of Salvia aratocensis Extract obtained by Supercritical Fluid Extraction and its Antigenotoxicity against Ultraviolet Radiation-Induced DNA Damage
    (2015) Fuentes Lorenzo, Jorge Luis; Stashenko, Elena E.; Quintero Ruiz, Nathalia
    Salvia is a cosmopolitan and important genus, widely used in flavoring and traditional medicine for treatments of respiratory and gastrointestinal diseases. The present work evaluated the chemical composition of Salvia aratocensis extract obtained by supercritical CO2 extraction, as well as its DNA protective effect against ultraviolet radiation (UVR)-induced genotoxicity. S. aratocensis extract composition was determined by gas chromatography-mass spectrometry (GCMS). The antigenotoxic effect of S. aratocensis extract was assayed in co-incubation procedures using the SOS chromotest in Escherichia coli PQ37 cells. The major components of the plant extract were α-cadinol (31.8%), 1,10-epi-cubenol (12.9%), trans-β-caryophyllene (7.7%), -cadinene (6.6%), and squalene (6.5%). S. aratocensis extract protected bacterial cells against UVR-induced genotoxicity at doses between 0.063 and 1 mg/mL. The results were discussed in relation to the DNA protective potential of the S. aratocensis extract and its major constituents.
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    Survival and SOS Response Induction in Ultraviolet B irradiated Escherichia coli Cells Defective to DNA Repair Mechanisms
    (2014-09) Prada Medina, César Augusto; Aristizabal Tessmer, Elke Tatjana; Quintero Ruiz, Nathalia; Serment Guerrero, Jorge Humberto; Fuentes Lorenzo, Jorge Luis
    Escherichia coli react to ultraviolet radiation by activating a cellular response against genotoxic stress known as SOS response. In this paper, the importance of different gene products involved in DNA repair to both survival and SOS activation in E. coli strains exposed to ultraviolet B radiation was evaluated. For this purpose, we determined cell survival through the plate counting method in E. coli strains carrying uvrA, oxyR, recO, recN, recJ, exoX, recB, recD, fpg and xonA mutations and in the wild type strain. These strains also contain the genetic sulA