Browsing by Author "Gualtero Escobar, Diego Fernando"

Now showing 1 - 6 of 6
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    Efecto de rosuvastatina sobre la expresión in vitro de pecam-1 en células endoteliales humanas estimuladas con los de porphyromonas gingivalis
    (2015) Buitrago Ramírez, Diana Marcela; Viafara, Sergio; Lafaurie Villamil, Gloria Inés; Gualtero Escobar, Diego Fernando
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    Inducción de disfunción endotelial in vitro por lipopolisacarido de bacterias periodontopaticas e inhibición de la inflamación por resolvina (rvd1) y estatina
    (2015-04) Lafaurie Villamil, Gloria Inés; Gualtero Escobar, Diego Fernando; Fontanilla Duque, Martha Raquel; Ebersole, Jeff; González Duque, Octavio Alberto; Romero, María Consuelo; De Ávila Quiroga, Juliette; Castillo Perdomo, Diana Marcela; Bernau Gutiérrez, Sebastián
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    Lipopolysaccharides isolated from Eikenella corrodens but not from Porphyromonas gingivalis W83 induce proatherosclerotic inflammatory responses in human coronary artery endothelial cells
    (2015) Gualtero Escobar, Diego Fernando; Bernau Gutiérrez, Sebastián; Viafara, Sergio; Buitrago Ramírez, Diana Marcela; Fontanilla Duque, Martha Raquel; Lafaurie Villamil, Gloria Inés
    Eikenella corrodens and Porphyromonas gingivalis are oral microorganisms associated with the periodontal disease and have been identified in atherosclerotic lesions. The pro-atherosclerotic potential of a periodontopathic species depends on the ability of the strain to infect the endothelium. Lipopolysaccharide (LPS) from atherosclerosis-associated bacteria causes innate inflammatory responses in the pathogenic processes induced by microorganisms. The purpose of this study was to compare the pro-inflammatory responses of human coronary artery endothelial cells (HCAECs) to LPS isolated from E. corrodens 23834 and P. gingivalis W83.
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    Memorias : XX Congreso Institucional de Investigaciones
    (2015) Amaya, Pilar; Aristizábal, Gerardo; Bermúdez, Claudia Priscila; Buitrago Ramírez, Diana Marcela; Calderón Robles, Justo Leonardo; Cardona Mendoza, Andrés Felipe; Castillo Perdomo, Diana Marcela; Castillo, Yormaris; Chila, Lorena; De Ávila Quiroga, Juliette; Delgadillo, Nathaly Andrea; Díaz, David; Durán Riveros, Juan Y.; Gómez, Luz Amparo; Gualtero Escobar, Diego Fernando; Gutiérrez Quintero, Juan G.; Lafaurie Villamil, Gloria Inés; Low-Calle, Ana María; Malagón, Clara; Martínez Valbuena, Carlos A.; Millán Ospina, Lina Viviana; Munévar Niño, Juan Carlos; Perdomo, Sandra; Rodríguez, Constanza; Romero, María Consuelo; Rosas, Jully Paola; Sabogal, María Alejandra; Torres, María Fernanda; Trujillo, Diego Alejandro; Vargas, Camilo Andrés; Viafara, Sergio
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    Purificación y caracterización de lipopolisacáridos de Eikenella corrodens 23834 y Porphyromonas gingivalis W83
    (2015) Gualtero Escobar, Diego Fernando; Porras Gaviria, Jeimy Paola; Bernau Gutiérrez, Sebastián; Buitrago Ramírez, Diana Marcela; Castillo Perdomo, Diana Marcela; Lafaurie Villamil, Gloria Inés
    Purification of lipopolysaccharide (LPS) or endotoxins and its characterization is an important aspect for studies aimed at clarify the role of these biomolecules from Gram negative bacteria present in the oral cavity and its relationship with periodontal and systemic diseases. This study describes an extraction, purification and characterization method of LPS from Eikenella corrodens 23834 and Porphyromonas gingivalis W83. LPS extraction was performed by using hot phenol-water; the purification was done with nuclease and protease enzymatic treatment, followed by size-exclusion chromatography (Sephacryl S-200 HR) with sodium deoxycholate as mobile phase. The characterization of the purified extracts was performed by spectrophotometric scanning, SDS-PAGE biochemical tests, Purpald assay and chromogenic LAL test. As control, commercial LPS from Escherichia coli, Salmonella typhimurium, P. gingivalis, and Rodobacter sphaeroides were used. The methodology mentioned above had allowed obtaining high purity LPS by identifying KDO or heptoses, a chemotype S-LPS (smooth) to E. corrodens; SR-LPS (semi-rough) for P. gingivalis W83. Both purified LPS showed endotoxic capacity at low valoraconcentrations. The methodology used in this study for purification and characterization of LPS from the whole bacteria was efficient when it was compared with commercial LPS.
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    Rosuvastatin inhibits IL-8 and IL-6 production in human coronary artery endothelial cells stimulated with Aggregatibacter actinomycetemcomitans
    (2015) Gualtero Escobar, Diego Fernando; Viafara, Sergio; Morantesa, Sandra J.; González Duque, Octavio Alberto; Lafaurie Villamil, Gloria Inés
    Aggregatibacter actinomycetemcomitans (A.a) is a Gram negative periodontopathogen that has been highly associated with endocarditis and atherosclerosis. However, the potential mechanisms by which A.a could be contributing to atherosclerosis remain unclear. The purpose of this study was to determine the effect of purified LPS from A.a (Aa-LPS) on the expression of pro-inflammatory molecules (i.e., adhesion molecules, Toll-like receptors and cytokines/chemokines) associated with the pathogenesis of atherosclerosis in human coronary artery endothelial cells (HCAECs), as well as evaluating the potential of Rosuvastatin (RSV) for inhibiting the A.a-induced endothelial responses. HCAECs were stimulated with purified A. a-LPS and cytokine expression levels determined by qPCR and flow cytometry, and TLR2 and TLR4 expression evaluated by ELISA fluorometric assay. The effect of RSV in Aa-LPS-induced pro-inflammatory responses was also studied using similar experimental approaches. A. a-LPS increased the expression of IL-6, IL-8, and TLR2 in HCAECs. No effects in the expression of adhesion molecules were observed. Aa-induced IL-6 and IL-8 production was inhibited by RSV particularly at higher doses. These results suggest that Aa-LPS plays a role in pro-inflammatory endothelial responses that could be contributing to the atherosclerotic process, and the use of statins (i.e., RSV) could be reducing the likelihood for Aa-induced pro-atherosclerotic endothelial responses.