Browsing by Author "Castillo Perdomo, Diana Marcela"

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    Inducción de disfunción endotelial in vitro por lipopolisacarido de bacterias periodontopaticas e inhibición de la inflamación por resolvina (rvd1) y estatina
    (2015-04) Lafaurie Villamil, Gloria Inés; Gualtero Escobar, Diego Fernando; Fontanilla Duque, Martha Raquel; Ebersole, Jeff; González Duque, Octavio Alberto; Romero, María Consuelo; De Ávila Quiroga, Juliette; Castillo Perdomo, Diana Marcela; Bernau Gutiérrez, Sebastián
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    Memorias : XX Congreso Institucional de Investigaciones
    (2015) Amaya, Pilar; Aristizábal, Gerardo; Bermúdez, Claudia Priscila; Buitrago Ramírez, Diana Marcela; Calderón Robles, Justo Leonardo; Cardona Mendoza, Andrés Felipe; Castillo Perdomo, Diana Marcela; Castillo, Yormaris; Chila, Lorena; De Ávila Quiroga, Juliette; Delgadillo, Nathaly Andrea; Díaz, David; Durán Riveros, Juan Y.; Gómez, Luz Amparo; Gualtero Escobar, Diego Fernando; Gutiérrez Quintero, Juan G.; Lafaurie Villamil, Gloria Inés; Low-Calle, Ana María; Malagón, Clara; Martínez Valbuena, Carlos A.; Millán Ospina, Lina Viviana; Munévar Niño, Juan Carlos; Perdomo, Sandra; Rodríguez, Constanza; Romero, María Consuelo; Rosas, Jully Paola; Sabogal, María Alejandra; Torres, María Fernanda; Trujillo, Diego Alejandro; Vargas, Camilo Andrés; Viafara, Sergio
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    Purificación y caracterización de lipopolisacáridos de Eikenella corrodens 23834 y Porphyromonas gingivalis W83
    (2015) Gualtero Escobar, Diego Fernando; Porras Gaviria, Jeimy Paola; Bernau Gutiérrez, Sebastián; Buitrago Ramírez, Diana Marcela; Castillo Perdomo, Diana Marcela; Lafaurie Villamil, Gloria Inés
    Purification of lipopolysaccharide (LPS) or endotoxins and its characterization is an important aspect for studies aimed at clarify the role of these biomolecules from Gram negative bacteria present in the oral cavity and its relationship with periodontal and systemic diseases. This study describes an extraction, purification and characterization method of LPS from Eikenella corrodens 23834 and Porphyromonas gingivalis W83. LPS extraction was performed by using hot phenol-water; the purification was done with nuclease and protease enzymatic treatment, followed by size-exclusion chromatography (Sephacryl S-200 HR) with sodium deoxycholate as mobile phase. The characterization of the purified extracts was performed by spectrophotometric scanning, SDS-PAGE biochemical tests, Purpald assay and chromogenic LAL test. As control, commercial LPS from Escherichia coli, Salmonella typhimurium, P. gingivalis, and Rodobacter sphaeroides were used. The methodology mentioned above had allowed obtaining high purity LPS by identifying KDO or heptoses, a chemotype S-LPS (smooth) to E. corrodens; SR-LPS (semi-rough) for P. gingivalis W83. Both purified LPS showed endotoxic capacity at low valoraconcentrations. The methodology used in this study for purification and characterization of LPS from the whole bacteria was efficient when it was compared with commercial LPS.