Browsing by Author "Castiblanco Rubio, Gina Alejandra"

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    Cartilla para odontólogos Diagnóstico diferencial entre fluorosis dental y caries
    (2015-11) Martignon Biermann, Stefania; Avila, Viviana; Castiblanco Rubio, Gina Alejandra; González Carrera, María Clara; Opazo Gutiérrez, Mario Omar; Martínez Mier, Esperanza Ángeles; Cury, Jaime Aparecido; Ruiz Carrizosa, Jaime Alberto; Silva Hermida, Blanca Cecilia
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    Diagnóstico diferencial entre caries y fluorosis: reproducibilidad entre examinadores en diagnostico visual y confirmacion histologica. (od124)
    (2014-10) Castiblanco Rubio, Gina Alejandra; Martignon Biermann, Stefania; Marín Gallón, Lina María; Martínez Mier, Esperanza Ángeles; Úsuga Vacca, Margarita V.
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    Diferencias en características demográficas, biológicas y sociales entre estadios de severidad de fluorosis dental en población preescolar y escolar de cuatro municipios del Huila
    (2015) Martignon Biermann, Stefania; Castiblanco Rubio, Gina Alejandra; Silva Hermida, Blanca Cecilia; Opazo Gutiérrez, Mario Omar; González Carrera, María Clara; Ávila, Viviana; Gamboa, Luis Fernando; Marín Gallón, Lina María; Cortés, Fabián; Ruiz Carrizosa, Jaime Alberto
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    Identification of proteins from human permanent erupted enamel
    (2015-09) Castiblanco Rubio, Gina Alejandra; Rutishauser, Dorothea; Ilag, Leopold L.; Martignon Biermann, Stefania; Castellanos, Jaime Eduardo; Mejía, Wilson
    Proteins from the extracellular matrix of enamel are highly specific and necessary for proper enamel formation. Most proteins are removed from the matrix by enamel proteases before complete mineralization is achieved; however, some residual protein fragments persist in the mineralized matrix of erupted enamel. So far, only amelogenin peptides obtained by traditional bottom-up proteomics have been recovered and identified in human permanent erupted enamel. In this study, we hypothesize that other enamel-specific proteins are also found in human permanent enamel, by analysing human erupted third molars. Pulverized enamel was used to extract proteins, and the protein extract was subjected directly to liquid-chromatography coupled to tandem mass spectrometry (LC-MS/MS) without a previous trypsindigestion step. Amelogenin and non-amelogenin proteins (ameloblastin and enamelin) were succesfully identified. The sequences of the naturally occurring peptides of these proteins are reported, finding in particular that most of the peptides from the amelogenin X-isoform come from the tyrosine-rich amelogenin peptide (TRAP) and that some were identified in all specimens. In conclusion, our LC-MS/MS method without trypsin digestion increased the coverage of identification of the enamel proteome from a few amelogenin peptides to a higher number of peptides from three enamel-specific proteins.
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    In vitro validation of Quantitative Light-induced Fluorescence (QLF) for the diagnosis of enamel fluorosis in permanent teeth
    (2015) Cuevas Espinosa, Diana Milena; Martínez Mier, Esperanza Ángeles; Masatoshi, Ando; Castiblanco Rubio, Gina Alejandra; Cortés, Fabián; Rincón Bermúdez, Claudia Milena; Martignon Biermann, Stefania
    The use of Quantitative Light-induced Fluorescence (QLF) for the diagnosis of enamel fluorosis has not been validated. This study aimed to validate QLF as a diagnostic tool for mild and moderate fluorosis in permanent teeth, comparing it to visual diagnosis and histological assessment completed using polarized light microscopy (PLM). The buccal surfaces of 139 teeth were visually classified using the Thylstrup and Fejerskov Index (TFI) into sound (TFI 0; n=17), mild (TFI 1-2; n=69) and moderate (TFI 3-4; n=43) fluorosis. Fluorosis was then assessed with QLF using the entire surface and a region of interest (ROI), identified as the most representative region of a fluorosis lesion. PLM images of longitudinal thin sections including the ROI were assessed for histological changes. Correlations among TFI, PLM, and QLF were determined. A ROC curve was conducted to determine QLF’s diagnostic accuracy when compared to the TFI and PLM assessments. This was used to assess the probability that the images were correctly ranked according to severity as determined by PLM and TFI. A positive correlation was found between QLF and PLM, and between QLF and TFI. QLF showed highest sensitivity and specificity for the diagnosis of mild fluorosis. There was also a strong agreement between TFI and PLM. The selection of a ROI resulted in a stronger correlation with TFI and PLM than when the entire surface was used. The study results indicate that defining a ROI for QLF assessments is a valid method for the diagnosis of mild and moderate enamel fluorosis.
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    Quantitative light fluorescence (QLF) assessment of mild and moderate enamel fluorosis in primary teeth
    (2015) Rincón Bermúdez, Claudia Milena; Martínez Mier, Esperanza Ángeles; Castiblanco Rubio, Gina Alejandra; Masatoshi, Ando; Cortés, Fabián; Cuevas Espinosa, Diana Milena; Martignon Biermann, Stefania
    Enamel fluorosis affects both permanent and primary teeth; however, there are few studies on primary teeth. Fluorosis in primary teeth is difficult to diagnose for mild and moderate cases. Quantitative light-induced fluorescence (QLF) has been used for enamel fluorosis on the permanent dentition, but there are no current studies on primary fluorotic teeth. The purpose of this study was to assess mild to moderate enamel fluorosis in primary teeth using QLF. The buccal surfaces of exfolicated and extracted primary teeth (n=113) were visually examined and classified using the Thylstrup & Fejerskov Index (TFI) for fluorosis into categories TFI-0 to TFI-4. Fluorescence images were acquired. Image analysis was performed using the complete tooth surface (S) and through the selection of a Region of Interest (ROI), which was determined as the region where the fluorosis lesions were more representative and with at least a minimum of adjacent sound area. Area (mm2), fluorescence loss (%) and ΔQ (mm2×%) values were obtained. The correlation between QLF variables and visual diagnosis was determined using the Kendall-tau coefficient. Sencitivity, specificity, and Receiver Operating Characteristic analysis was performed for QLF versus visual diagnosis. Moderate to strong positive correlation was found between QLF and visual diagnosis using the ROI. QLF showed greater sensitivity and specificity for the diagnosis of mild fluorosis than moderate fluorosis and with the ROI analysis than with S. QLF was a useful tool for the assessment of mild and moderate fluorosis in primary teeth using a ROI analysis technique.